Introduction
Human placental amnion (AM) has a long history in the field of wound treatment. Advances in tissue preservation have helped to overcome the short shelf life of fresh AM and led to the commercialization of amnion products. Viable cryopreserved amniotic membrane (VCAM), which retains all components of fresh AM, has shown positive outcomes in wound management. However, cryopreservation requires specialized ultra-low temperature storage equipment, thus limiting widespread use of VCAM. Recently, a novel lyopreservation technology has been developed that allows for ambient storage of living cells and tissues.
Purpose of Study
The structural, cellular viability, and wound-relevant properties of viable lyopreserved AM (VLAM) were investigated in vitro and in in vivo diabetic mouse wound models.
About VLAM
VLAM retains the native components of placental tissue, including:
- extracellular matrix (ECM)
- endogenous growth factors, and
- viable cells including fibroblast, epithelial cells, and mesenchymal stem cells (MSCs)
VLAM is stored at ambient temperatures and offers ease of application
Conclusion
- VLAM’s structural, cellular viability, and functional properties are equivalent to VCAM
- VLAM is stored at ambient temperatures making it more accessible for widespread use
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